Article
Mutational analysis of the role of the N terminus of actin in actomyosin interactions. Comparison with other mutant actins and implications for the cross-bridge cycle.
Biochemistry - 24 Dec 1996
Miller C J, Wong W W, Bobkova E, Rubenstein P A, Reisler E
Abstract excerpt
Yeast actin mutants with acidic residues at the N terminus either neutralized (DNEQ) or deleted (delta-DSE) were used to assess the role of N-terminal acidic residues in the interactions of actin with myosin in the contractile cycle. Cosedimentation experiments revealed an approximately 3-fold de...
Topics
- Actins
- Actomyosin
- Adenosine Triphosphate
- Amino Acid Sequence
- Animals
- Binding Sites
- In Vitro Techniques
- Models, Molecular
- Muscle Contraction
- Mutation
- Myosin Subfragments
- Protein Binding
- Protein Conformation
- Rabbits
- Saccharomyces cerevisiae
- Sequence Deletion
- Static Electricity