Article
Rational construction of rpsL or/and rpoB merodiploids affects biosynthesis of secondary metabolite in Streptomyces.
Molecular biology reports - 5 May 2026
Lu Chen, Pan Xiaoyu, Yang Shiying, Zhang Jinyao, Jiang Yujie, Zhang Yongyong, Bechthold Andreas, Ma Zheng
Abstract excerpt
BACKGROUND: Ribosome engineering technology typically involves resistance screening of streptomycin and rifampin to introduce point mutations in genes ribosomal protein S12 (rpsL) or RNA polymerase (rpoB), thereby enhancing the synthesis level of secondary metabolites in Streptomyces. Currently, directly introducing copies of genes rpsL and rpoB carrying "beneficial mutations" to construct diploids has become a...
Topics
- DNA-Directed RNA Polymerases
- Streptomyces
- Ribosomal Proteins
- Bacterial Proteins
- Secondary Metabolism
- Benzoisochromanequinones
- Mutation
- Anthraquinones
- Streptomyces coelicolor
- Ribosomes