Article
Metal binding affinity and structural properties of calmodulin-like protein 14 from Arabidopsis thaliana.
Protein science : a publication of the Protein Society - 1 Aug 2016
Vallone Rosario, La Verde Valentina, D'Onofrio Mariapina, Giorgetti Alejandro, Dominici Paola, Astegno Alessandra
Abstract excerpt
In addition to the well-known Ca(2+) sensor calmodulin, plants possess many calmodulin-like proteins (CMLs) that are predicted to have specific roles in the cell. Herein, we described the biochemical and biophysical characterization of recombinant Arabidopsis thaliana CML14. We applied isothermal titration calorimetry to analyze the energetics of Ca(2+) and Mg(2+) binding to CML14, and nuclear magnetic resonance...
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