Article
Analysis of the reaction mechanism of the non-specific endonuclease of Serratia marcescens using an artificial minimal substrate.
FEBS letters - 18 Nov 1996
Kolmes B, Franke I, Friedhoff P, Pingoud A
Abstract excerpt
We have studied the mechanism of action of the Serratia nuclease using deoxythymidine 3',5'-bis-(p-nitrophenyl-phosphate) as a substrate. A comparison of the activity with which the wild-type enzyme and several mutant enzymes attack this artificial substrate and herring sperm DNA, respectively, s...
Topics
- DNA
- Edetic Acid
- Endodeoxyribonucleases
- Endoribonucleases
- Hydrolysis
- Kinetics
- Magnesium
- Mutation
- Nitrophenols
- Serratia marcescens
- Thymine Nucleotides