Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp. PCC 7120 endonuclease NucA and its inhibition by NuiA.

doi:10.1006/jmbi.2000.3586

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Article

Mechanism of DNA cleavage by the DNA/RNA-non-specific Anabaena sp. PCC 7120 endonuclease NucA and its inhibition by NuiA.

Journal of molecular biology - 24 Mar 2000

Meiss G, Gimadutdinow O, Haberland B, Pingoud A

Abstract excerpt

A structural model of the DNA/RNA non-specific endonuclease NucA from Anabaena sp. PCC7120 that has been obtained on the basis of the three-dimensional structure of the related Serratia nuclease, suggests that the overall architecture of the active site including amino acid residues H124, N155 and E163 (corresponding to H89, N119 and E127 in Serratia nuclease) is similar in both nucleases. Substitution of these...

Topics

Amino Acid Sequence
Amino Acid Substitution
Anabaena
Bacterial Proteins
Binding Sites
Catalytic Domain
Circular Dichroism
DNA
Dimerization
Endonucleases
Glutamic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hydrolysis

Kinetics

Models, Chemical

Models, Molecular

Molecular Sequence Data

Mutation

Phosphodiesterase Inhibitors

Source

Open in PubMed

Identifiers

PMID: 10715218
DOI: 10.1006/jmbi.2000.3586

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