Article
Mutations in an Abf1p binding site in the promoter of yeast RPO26 shift the transcription start sites and reduce the level of RPO26 mRNA.
Yeast (Chichester, England) - 1 Oct 1996
Nouraini S, Hu J, McBroom L D, Friesen J D
Abstract excerpt
A binding site for the transcription factor Abf1p was identified as an important promoter element of the gene that encodes Rpo26, a subunit common to all three yeast nuclear RNA polymerases (RNAP). Mutations in the Abf1p binding site were identified among a pool of rpo26 mutant alleles that confe...
Topics
- Alleles
- Base Sequence
- Binding Sites
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Gene Expression Regulation, Fungal
- Molecular Sequence Data
- Mutagenesis
- Nucleic Acid Hybridization
- Plasmids
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- RNA, Messenger
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors
- Transcriptional Activation