Chromosomal replication incompatibility in Dam methyltransferase deficient Escherichia coli cells.

Article

Chromosomal replication incompatibility in Dam methyltransferase deficient Escherichia coli cells.

The EMBO journal - 1 Nov 1996

Løbner-Olesen A, von Freiesleben U

Abstract excerpt

Dam methyltransferase deficient Escherichia coli cells containing minichromosomes were constructed. Free plasmid DNA could not be detected in these cells and the minichromosomes were found to be integrated in multiple copies in the origin of replication (oriC) region of the host chromosome. The a...

Topics

Chromosomes, Bacterial
DNA Methylation
DNA Replication
DNA, Bacterial
Escherichia coli
Escherichia coli Proteins
Genes, Bacterial
Mutation
Plasmids
Replication Origin
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Transformation, Genetic

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