Article
A high frequency of defective vif genes in peripheral blood mononuclear cells from HIV type 1-infected individuals.
AIDS research and human retroviruses - 1 Nov 1996
Tominaga K, Kato S, Negishi M, Takano T
Abstract excerpt
We studied the heterogeneity and intactness of the vif gene in six HIV-1-infected individuals at various clinical stages. The proviral vif sequences in peripheral blood mononuclear cells were amplified by PCR, followed by cloning and sequencing of 45 vif clones. The intraindividual diversity of t...
Topics
- Adult
- Amino Acid Sequence
- Cloning, Molecular
- Female
- Genes, vif
- Genetic Variation
- HIV Infections
- HIV-1
- Humans
- Leukocytes, Mononuclear
- Male
- Middle Aged
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Sequence Homology, Amino Acid