Article
Growth and propagation of normal rat intestinal epithelial cells.
In vitro cellular & developmental biology. Animal - 1 Feb 1996
Odedra R M, Hart C A, Saunders J R, Getty B, van de Wall S, Sorensen S H, Embaye H, Batt R M
Abstract excerpt
A combination of mild proteolytic digestion and selective growth stimulation has been used to isolate and propagate adult rat intestinal epithelial cells with a finite life span. Growth of these cells on a variety of matrices and on mesenchymal cells has resulted in the expression of brush border enzymes including sucrase-isomaltase, aminopeptidase N, and alkaline phosphatase. Examination of the cells at the...
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