Article
Development and characterization of polarized primary cultures of rat intrahepatic bile duct epithelial cells.
Laboratory investigation; a journal of technical methods and pathology - 1 Jan 1996
Vroman B, LaRusso N F
Abstract excerpt
The study of intrahepatic bile duct epithelial cells (i.e., cholangiocytes) has been limited by the lack of a polarized in vitro model that allows easy access to both apical and basolateral cell surfaces. Therefore, we developed a cell line of polarized normal rat cholangiocytes (NRCs) and established conditions that produced a confluent monolayer of cells grown on collagen-coated filters of tissue culture...
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