Article
Single amino acid mutations in transmembrane domain 5 confer to the plasma membrane Ca2+ pump properties typical of the Ca2+ pump of endo(sarco)plasmic reticulum.
The Journal of biological chemistry - 6 Oct 2000
Guerini D, Zecca-Mazza A, Carafoli E
Abstract excerpt
Conserved residues in some of the transmembrane domains are proposed to mediate ion translocation by P-type pumps. The plasma membrane Ca(2+) pump (PMCA) lacks 2 of these residues in transmembrane domains (TM) 5 and 8. In particular, a glutamic acid (Glu-771) residue in TM5, which is proposed to be involved in the binding and transport of Ca(2+) by the sarcoplasmic reticulum Ca(2+) pump (SERCA), is replaced by an...
Topics
- Animals
- COS Cells
- Calcium
- Calcium-Transporting ATPases
- Cation Transport Proteins
- Cell Line
- Cell Membrane
- DNA, Complementary
- Dose-Response Relationship, Drug
- Endoplasmic Reticulum
- Fluorescent Antibody Technique, Indirect
- Genetic Vectors
- Glutamic Acid