Article
Mild RIP-an alternative method for in vivo mutagenesis of the albino-3 gene in Neurospora crassa.
Molecular & general genetics : MGG - 25 Sept 1996
Barbato C, Calissano M, Pickford A, Romano N, Sandmann G, Macino G
Abstract excerpt
We have used a biological phenomenon that occurs in Neurospora crassa, termed Repeat-Induced Point mutation (RIP), to create partially functional mutant alleles of the albino-3 (al-3) gene encoding geranylgeranyl pyrophosphate synthase, an enzyme involved in the biosynthesis of carotenoids and diverse prenylated compounds. A total of 70 RIP-induced al-3 mutants were identified by their pale albino phenotype,...
Topics
- Alkyl and Aryl Transferases
- Amino Acid Sequence
- Carotenoids
- Cloning, Molecular
- Gene Expression Regulation, Fungal
- Genetic Techniques
- Geranylgeranyl-Diphosphate Geranylgeranyltransferase
- Molecular Sequence Data
- Mutagenesis
- Mutation