Article
Mild RIP-an alternative method for in vivo mutagenesis of the albino-3 gene in Neurospora crassa.
Molecular & general genetics : MGG - 25 Sept 1996
Barbato C, Calissano M, Pickford A, Romano N, Sandmann G, Macino G
Abstract excerpt
We have used a biological phenomenon that occurs in Neurospora crassa, termed Repeat-Induced Point mutation (RIP), to create partially functional mutant alleles of the albino-3 (al-3) gene encoding geranylgeranyl pyrophosphate synthase, an enzyme involved in the biosynthesis of carotenoids and diverse prenylated compounds. A total of 70 RIP-induced al-3 mutants were identified by their pale albino phenotype,...
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