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Article

Generation of new mutants of nmr, the negative-acting nitrogen regulatory gene of Neurospora crassa, by repeat induced mutation.

Current genetics - 1 Sept 1991

Jarai G, Marzluf G A

Abstract excerpt

The repeat induced point mutation (RIP) phenomenon has been used to generate new mutants of nmr, the negative nitrogen regulatory gene in Neurospora crassa. The wild-type nmr gene was cotransformed along with the hygromycin B resistance gene into wild-type cells by selecting for hygromycin B resistance. Following purification of primary transformants using microconidia, many chlorate-sensitive progeny were...

Topics

Amino Acid Sequence
Base Sequence
Blotting, Southern
Genes, Regulator
Molecular Sequence Data
Mutation
Neurospora crassa
Nitrogen
Phenotype
Plasmids
Polymerase Chain Reaction

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PMID: 1834354
DOI: 10.1007/BF00318516

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1834354-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Generation of new mutants of nmr, the negative-acting nitrogen regulatory gene of Neurospora crassa, by repeat induced mutation.PMID 1834354Read ↗

Analysis of conventional and in vitro generated mutants of nmr, the negatively acting nitrogen regulatory gene of Neurospora crassa.Semantic proximity Read ↗

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