Article
Electric-field-induced Schiff-base deprotonation in D85N mutant bacteriorhodopsin.
Proceedings of the National Academy of Sciences of the United States of America - 15 Oct 1996
Kolodner P, Lukashev E P, Ching Y C, Rousseau D L
Abstract excerpt
The application of an external electric field to dry films of Asp-85-->Asn mutant bacteriorhodopsin causes deprotonation of the Schiff base, resulting in a shift of the optical absorption maximum from 600 nm to 400 nm. This is in marked contrast to the case of wild-type bacteriorhodopsin films, in which electric fields produce a red-shifted product whose optical properties are similar to those of the acid-blue...
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