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Article

Electric-field effects in dry films of D85N and D85,96N mutant bacteriorhodopsin.

Bioelectrochemistry (Amsterdam, Netherlands) - 1 Feb 2000

Kolodner P, Lukashev E P, Ching Y C

Abstract excerpt

In the D85N mutant of the protein bacteriorhodopsin (BR), the Schiff base, by which the retinal chromophore is bound to the protein, exhibits an abnormally low proton affinity (pKa approximately 8.9). Recent experiments on thin films of this protein have shown that this causes the protonation state of the Schiff base, and thus the visible absorption spectrum, to be sensitive to external electric fields. In this...

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PMID: 10790781
DOI: 10.1016/s0302-4598(99)00071-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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