Structure changes upon deprotonation of the proton release group in the bacteriorhodopsin photocycle.

Abstract excerpt

In the photocycle of bacteriorhodopsin at pH 7, a proton is ejected to the extracellular medium during the protonation of Asp-85 upon formation of the M intermediate. The group that releases the ejected proton does not become reprotonated until the prephotolysis state is restored from the N and O intermediates. In contrast, at acidic pH, this proton release group remains protonated to the end of the cycle....

Topics

• Aspartic Acid
• Bacteriorhodopsins
• Hydrogen-Ion Concentration
• Mutation
• Photolysis
• Protons
• Spectrum Analysis