Temperature-sensitive mutations in the protein kinase-1 (pk-1) gene of the Autographa californica nuclear polyhedrosis virus that block very late gene expression.

Abstract excerpt

We have developed a genetic screen for temperature-sensitive mutations in the very late transcription apparatus of the Autographa californica nuclear polyhedrosis virus. This method starts with the BacPAKS virus, which has the Escherichia coli lacZ gene under the control of the very late polyhedrin promoter. The desired mutants are temperature-sensitive for beta-galactosidase production and can be complemented by...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Amino Acid Sequence
• Share to Base Sequence