Article
Temperature-sensitive mutations in the protein kinase-1 (pk-1) gene of the Autographa californica nuclear polyhedrosis virus that block very late gene expression.
Virology - 1 Oct 1996
Fan X, Thirunavukkarasu K, Weaver R F
Abstract excerpt
We have developed a genetic screen for temperature-sensitive mutations in the very late transcription apparatus of the Autographa californica nuclear polyhedrosis virus. This method starts with the BacPAKS virus, which has the Escherichia coli lacZ gene under the control of the very late polyhedr...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Cell Line
- Chromosome Mapping
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Gene Expression Regulation, Viral
- Genes, Viral
- Molecular Sequence Data
- Mutation
- Nucleopolyhedroviruses
- Protein Kinases
- Spodoptera
- Temperature
- Transcription, Genetic