Article
A temperature-sensitive IE1 protein of Autographa californica nuclear polyhedrosis virus has altered transactivation and DNA binding activities.
Virology - 10 May 1995
Choi J, Guarino L A
Abstract excerpt
The temperature-sensitive mutant tsB821 of Autographa californica nuclear polyhedrosis virus has two nucleotide transitions in the transregulatory ie1 gene (Ribiero et al. (1994) J. Virol. 68, 1075-1084). These mutations result in two conservative amino acid substitutions in the encoded protein. To determine whether the mutations affect the ability of IE1 to interact with viral enhancer elements, electrophoretic...
Topics
- Animals
- Base Sequence
- Cell Line
- DNA Primers
- DNA, Viral
- DNA-Binding Proteins
- Gene Expression
- Immediate-Early Proteins
- Molecular Sequence Data
- Mutation
- Nucleopolyhedroviruses