Article
Establishment of permanent human endothelial cells achieved by transfection with SV40 large T antigen that retain typical phenotypical and functional characteristics.
In vitro cellular & developmental biology. Animal - 1 Jan 1996
Moldovan F, Benanni H, Fiet J, Cussenot O, Dumas J, Darbord C, Soliman H R
Abstract excerpt
The plasmid pMK16 containing-SV40 replicated origin defective gene was efficiently introduced into early-passage human umbilical vein endothelial cells (HUVEC) using positively charged liposomes. The resulting cell line acquired an almost infinite lifespan, was morphologically unchanged, expresse...
Topics
- Antigens, Polyomavirus Transforming
- Arginine
- Biological Transport
- Cell Differentiation
- Cells, Cultured
- Endothelium, Vascular
- Humans
- Phenotype
- Sodium
- Transfection
- Umbilical Veins