Article
Establishment of permanent human endothelial cells achieved by transfection with SV40 large T antigen that retain typical phenotypical and functional characteristics.
In vitro cellular & developmental biology. Animal - 1 Jan 1996
Moldovan F, Benanni H, Fiet J, Cussenot O, Dumas J, Darbord C, Soliman H R
Abstract excerpt
The plasmid pMK16 containing-SV40 replicated origin defective gene was efficiently introduced into early-passage human umbilical vein endothelial cells (HUVEC) using positively charged liposomes. The resulting cell line acquired an almost infinite lifespan, was morphologically unchanged, expressed SV40-antigen, and coexpressed von Willebrand factor (vWF), tissue plasminogen activator (t-PA), plasminogen activator...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.