Article
The identification, cloning and mutagenesis of a genetic locus required for lipopolysaccharide biosynthesis in Bordetella pertussis.
Molecular microbiology - 1 Jan 1996
Allen A, Maskell D
Abstract excerpt
Bordetella pertussis lipopolysaccharide (LPS) is biologically active, being both toxic and immunogenic. Using transposon mutagenesis we have identified a genetic locus required for the biosynthesis of LPS in B. pertussis, which has been cloned and sequenced. We have also identified equivalent loc...
Topics
- Amino Acid Sequence
- Blotting, Western
- Bordetella
- Cloning, Molecular
- DNA Transposable Elements
- Deoxy Sugars
- Electrophoresis, Polyacrylamide Gel
- Lipopolysaccharides
- Molecular Sequence Data
- Molecular Structure
- Mutagenesis, Insertional
- Open Reading Frames
- Phenotype
- Polysaccharides
- Sequence Analysis