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Inactivation of formyltransferase (wbkC) gene generates a Brucella abortus rough strain that is attenuated in macrophages and in mice.

Vaccine - 2 Aug 2010

Lacerda Thaís Lourdes Santos, Cardoso Patrícia Gomes, Augusto de Almeida Leonardo, Camargo Ilana Lopes Baratella da Cunha, Afonso Daniela Almeida Freitas, Trant Cyntia Cardoso, Macedo Gilson Costa, Campos Eleonora, Cravero Silvio L, Salcedo Suzana P, Gorvel Jean-Pierre, Oliveira Sérgio Costa

Abstract excerpt

Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusDeltawbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-alpha when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in...

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PMID: 20580469
DOI: 10.1016/j.vaccine.2010.06.023

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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