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Article

Identification and cloning of waaF (rfaF) from Bordetella pertussis and use to generate mutants of Bordetella spp. with deep rough lipopolysaccharide.

Journal of bacteriology - 1 Jan 1998

Allen A G, Isobe T, Maskell D J

Abstract excerpt

A DNA locus from Bordetella pertussis capable of reconstituting lipopolysaccharide (LPS) O-antigen biosynthesis in Salmonella typhimurium SL3789 (rfaF511) has been isolated, by using selection with the antibiotic novobiocin. DNA within the locus encodes a protein with amino acid sequence similari...

Topics

Amino Acid Sequence
Bordetella
Bordetella pertussis
Cloning, Molecular
Genes, Bacterial
Genetic Complementation Test
Glycosyltransferases
Lipopolysaccharides
Molecular Sequence Data
Mutation
O Antigens

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Sequence Analysis, DNA

Species Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 9422589
DOI: 10.1128/JB.180.1.35-40.1998
PMCID: PMC106845

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9422589-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Identification and cloning of waaF (rfaF) from Bordetella pertussis and use to generate mutants of Bordetella spp. with deep rough lipopolysaccharide.PMID 9422589Read ↗

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