Genetic and biochemical analysis of the cysteinyl residues of isopenicillin N synthase from Streptomyces clavuligerus.

doi:10.1139/m96-112

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Article

Genetic and biochemical analysis of the cysteinyl residues of isopenicillin N synthase from Streptomyces clavuligerus.

Canadian journal of microbiology - 1 Aug 1996

Durairaj M, Leskiw B K, Jensen S E

Abstract excerpt

Isopenicillin N synthase (IPNS) from Streptomyces clavuligerus catalyses the oxidative cyclization of the acyclic tripeptide delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine into isopenicillin N. All four of the cysteine residues found in this enzyme were mutated individually into serine residues...

Topics

Base Sequence
Cysteine
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Oxidoreductases
Streptomyces
Structure-Activity Relationship

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