Article
Promoter elements that mediate the pH response of PCK mRNA in LLC-PK1-F+ cells.
The American journal of physiology - 1 Aug 1996
Holcomb T, Liu W, Snyder R, Shapiro R, Curthoys N P
Abstract excerpt
The onset of metabolic acidosis causes an increased transcription of the renal phosphoenolpyruvate carboxykinase (PCK) gene. When transgenic mice carrying a bovine growth hormone (bGH) gene driven by the -460 to +73 segment of the PCK promoter were made chronically acidotic, the bGH mRNA was incr...
Topics
- Animals
- Blotting, Northern
- Cattle
- Chloramphenicol O-Acetyltransferase
- Gene Deletion
- Growth Hormone
- Hydrogen-Ion Concentration
- Kidney
- LLC-PK1 Cells
- Male
- Mice
- Mice, Transgenic
- Mutation
- Phosphoenolpyruvate Carboxykinase (GTP)
- Promoter Regions, Genetic
- RNA, Messenger
- Swine
- Transfection