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Making Antibodies by Phage Display Technology

1 Apr 1994

Abstract excerpt

Antibody fragments of predetermined binding specificity have recently been constructed from repertoires of antibody V genes, bypassing hybridoma technology and even immunization. The V gene repertoires are harvested from populations of lymphocytes, or assembled in vitro, and cloned for display of associated heavy and light chain variable domains on the surface of filamentous bacteriophage. Rare phage are selected...

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PMID: 8011287
DOI: 10.1146/annurev.iy.12.040194.002245

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