Article
Defect in general priming conferred by linker region mutants of Escherichia coli dnaB.
Journal of bacteriology - 1 Aug 1996
Stordal L, Maurer R
Abstract excerpt
The dnaB gene of Escherichia coli encodes a bifunctional primase accessory protein/helicase necessary for chromosomal replication. Monomers of DnaB comprise two trypsin-resistant domains connected by a 45-amino-acid linker. To investigate the role of the linker in the structure and function of DnaB, we have purified and characterized three DnaB mutant proteins having single amino acid substitutions in the linker....
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