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Article

Quantitative determination of rare mRNA species by PCR and solid-phase minisequencing.

PCR methods and applications - 1 May 1992

Ikonen E, Manninen T, Peltonen L, Syvänen A C

Abstract excerpt

We present a new method for quantification of mRNA, in which the limitations of the current quantitative PCR methods can be overcome. A known amount of a synthetic RNA standard differing from the mRNA to be quantified by a single nucleotide is reverse-transcribed and amplified together with the mRNA template using a biotinylated primer. The biotinylated PCR product is immobilized on a streptavidin-coated solid...

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PMID: 1477657
DOI: 10.1101/gr.1.4.234

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AGA

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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