Article
Quantitative determination of rare mRNA species by PCR and solid-phase minisequencing.
PCR methods and applications - 1 May 1992
Ikonen E, Manninen T, Peltonen L, Syvänen A C
Abstract excerpt
We present a new method for quantification of mRNA, in which the limitations of the current quantitative PCR methods can be overcome. A known amount of a synthetic RNA standard differing from the mRNA to be quantified by a single nucleotide is reverse-transcribed and amplified together with the mRNA template using a biotinylated primer. The biotinylated PCR product is immobilized on a streptavidin-coated solid...
Topics
- Aspartylglucosaminuria
- Aspartylglucosylaminase
- Base Sequence
- Cells, Cultured
- Gene Expression Regulation, Enzymologic
- Genotype
- Humans
- Metabolism, Inborn Errors
- Molecular Sequence Data
- Organ Specificity
- Polymerase Chain Reaction