Article
Illegitimate transcription. Application to the analysis of truncated transcripts of the dystrophin gene in nonmuscle cultured cells from Duchenne and Becker patients.
The Journal of clinical investigation - 1 Oct 1991
Chelly J, Gilgenkrantz H, Hugnot J P, Hamard G, Lambert M, Récan D, Akli S, Cometto M, Kahn A, Kaplan J C
Abstract excerpt
We have previously demonstrated that there is a low level of transcription of tissue-specific genes in every cell type. In this study, we have taken advantage of this phenomenon, called illegitimate transcription, to analyze the muscle-type dystrophin mRNA in easily accessible cells such as lymphoid cells, fibroblasts, and peripheral blood cells from Duchenne and Becker muscular dystrophies with known internal...
Topics
- Base Sequence
- Cells, Cultured
- Dystrophin
- Humans
- Molecular Sequence Data
- Muscular Dystrophies
- Mutation
- Phenotype
- Polymerase Chain Reaction
- RNA Splicing