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Ultrasensitive quantification of multiplexed mRNA variants via splice-junction anchored DNA probes and SplintR ligase-initiated PCR.

Chemical communications (Cambridge, England) - 30 Sept 2021

Jia Yuting, Han Jun, Wang Hui, Hong Weixiang, Wang Honghong, Zhang Mai, Li Zhengping

Abstract excerpt

A method based on mRNA-templated ligation of splice-junction anchored DNA probes followed by PCR amplification of the ligated product has been developed for multiplexed detection of mRNA splice variants with high sensitivity and specificity. The proposed assay can detect as low as 10 aM mRNA splicing variants and has been successfully applied to detect real samples.

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PMID: 34498616
DOI: 10.1039/d1cc03033g

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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