Article
Location and domain structure of Escherichia coli ribosomal protein L7/L12: site specific cysteine crosslinking and attachment of fluorescent probes.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 Jan 2000
Traut R R, Dey D, Bochkariov D E, Oleinikov A V, Jokhadze G G, Hamman B, Jameson D
Abstract excerpt
Five different variants of L7/L12 containing single cysteine substitutions, two in the N-terminal (NTD) and three in the C-terminal domain (CTD), were produced, modified with [125I]N-[4-(p-azidosalicylamido)butyl]-3-(2'-pyridyldithio) propionamide ([125I]APDP), a sulfhydryl-specific, heterobifunctional, cleavable photo-cross-linking reagent, and reconstituted into ribosomes. These were irradiated, the total...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
