Article
Structural and functional domains of Escherichia coli ribosomal protein L7/L12. The hinge region is required for activity.
The Journal of biological chemistry - 15 Jan 1993
Oleinikov A V, Perroud B, Wang B, Traut R R
Abstract excerpt
Variant forms of Escherichia coli ribosomal protein L7/L12 were constructed, overexpressed, and purified. These included proteins that deleted residues 35-52 (delta 35-52) and 42 to 52 (delta 42-52), others that contained single cysteine substitutions at residues 63 and 89, and combinations of th...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Chromatography, Gel
- Cloning, Molecular
- Cysteine
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Bacterial
- Genetic Variation
- Iodoacetates
- Iodoacetic Acid
- Mutagenesis, Site-Directed
- Protein Conformation
- Recombinant Proteins
- Restriction Mapping
- Ribosomal Proteins
- Ribosomes
- Sequence Deletion