Article
Delta Lys120, a mutation which destabilizes the ribosome-binding domain of ribosomal protein L7/L12.
Biochemistry and cell biology = Biochimie et biologie cellulaire - 1 May 1990
Laughrea M, Higgins E
Abstract excerpt
Five-residue-long deletions centered on Ala63, Ala75, and Glu118 of ribosomal protein L7/L12 gave low mutant yields (5% or less) when the mutant genes were cloned in phage M13mp18 and controlled by the L10 promotor. Deletions of Glu118-Lys120 or Lys120 (the COOH-terminus of L7/L12) gave higher mu...
Topics
- Amino Acid Sequence
- Ammonium Chloride
- Base Sequence
- Binding Sites
- Centrifugation, Density Gradient
- Chlorides
- Chromosome Deletion
- Cloning, Molecular
- Electrophoresis
- Escherichia coli
- Ethanol
- Lithium
- Lithium Chloride
- Lysine
- Molecular Sequence Data
- Mutation
- Ribosomal Proteins
- Ribosomes