Analysis of strains with mutations in six genes encoding subunits of the V-ATPase: eukaryotes differ in the composition of the V0 sector of the enzyme.

Abstract excerpt

To address questions about the structure of the vacuolar ATPase, we have generated mutant strains of Neurospora crassa defective in six subunits, C, H, a, c, c', and c''. Except for strains lacking subunit c', the mutant strains were indistinguishable from each other in most phenotypic characteristics. They did not accumulate arginine in the vacuoles, grew poorly at pH 5.8 with altered morphology, and failed to...

Topics

• Amino Acid Sequence
• Base Sequence
• Gene Deletion
• Hydrogen-Ion Concentration
• Macrolides
• Molecular Sequence Data
• Mutation
• Neurospora crassa
• Phenotype
• Protein Structure, Tertiary