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Article

Domain analysis of human U5 RNA. Cap trimethylation, protein binding, and spliceosome assembly.

The Journal of biological chemistry - 2 Aug 1996

Hinz M, Moore M J, Bindereif A

Abstract excerpt

We have analyzed the sequence requirements of the human U5 RNA during small nuclear ribonucleoprotein (snRNP) and spliceosome assembly. A collection of mutant derivatives of the human U5 RNA gene was constructed in a U1 expression vector and transiently transfected in mammalian cells. Using immunoprecipitation and affinity selection assays, the cap trimethylation, the binding of Sm proteins and of the U5...

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Identifiers

PMID: 8702566
DOI: 10.1074/jbc.271.31.19001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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