Article
Chemostat selection of an Escherichia coli mutant containing permease with enhanced lactose affinity.
Biochemical and biophysical research communications - 16 Jul 1996
Tsen S D, Lai S C, Pang C P, Lee J I, Wilson T H
Abstract excerpt
Chemostats supplied with limited lactose were used to ask whether it was possible to generate and isolate any mutant of Escherichia coli lactose permease which allowed cells to grow faster. The permease and beta-galactosidase activities of the chemostat culture initially rose together to reach a...
Topics
- Bacteriological Techniques
- Base Sequence
- Cloning, Molecular
- DNA Primers
- Escherichia coli
- Escherichia coli Proteins
- Genotype
- Kinetics
- Lac Operon
- Membrane Transport Proteins
- Molecular Sequence Data
- Monosaccharide Transport Proteins
- Plasmids
- Polymerase Chain Reaction
- Substrate Specificity
- Symporters
- Time Factors