Article
Increase in the catalytic rate of beta-galactosidase by selection in chemostats at changing dilution rates.
Biochemical and biophysical research communications - 14 Feb 1990
Tsen S D
Abstract excerpt
The experiments presented in this paper explore whether mutants with catalytically more active beta-galactosidase (E.C. 3.2.1.23) can be selected in lactose-limited chemostats. This experimental system has been chosen because lactose metabolism in Escherichia coli is well understood both from a biochemical and genetic point of view. In a lactose-limited chemostat with constant dilution rates, both...
Topics
- Bacteriological Techniques
- Escherichia coli
- Escherichia coli Proteins
- Galactosidases
- Kinetics
- Membrane Transport Proteins
- Monosaccharide Transport Proteins
- Mutation
- Symporters
- beta-Galactosidase