Article
[Molecular mechanism of suppressing lambda N gene's expression in E. coli ribosomal protein S12 streptomycin-dependent mutant].
Yi chuan xue bao = Acta genetica Sinica - 1 Jan 1996
Jiang X, Weng M
Abstract excerpt
Using the polymerase chain reaction (PCR) method, the rpsLd gene was amplified and cloned, which encodes the streptomycin-dependent (Smd) mutant of ribosomal protein S12 in E.coli T83. The result of DNA sequencing showed an AAA to CAA mutation at codon 42, leading to the substitution of glutamine (Gln, Q) for lysine (Lys, K). According to the principle of Garnier, we predicted that there might be alterations in...
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