Article
Effect of 5'-proximal elements on decay of a model mRNA in Bacillus subtilis.
Molecular microbiology - 1 Jul 2005
Sharp Josh S, Bechhofer David H
Abstract excerpt
Previous work showed that a 42-nucleotide sequence from an SP82 bacteriophage early RNA functions as a 5' mRNA stabilizer in Bacillus subtilis. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis of decay of a model mRNA with alterations at the 5'-end was used to elucidate the mechanism of SP82-mediated stability. A predicted 5'-terminal stem-loop structure was essential for stabilization....
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