Article
Rapid burst kinetics in the hydrolysis of 4-nitrophenyl acetate by penicillin G acylase from Kluyvera citrophila. Effects of mutation F360V on rate constants for acylation and de-acylation.
The Biochemical journal - 1 Jun 1996
Roa A, Goble M L, García J L, Acebal C, Virden R
Abstract excerpt
The kinetics of release of 4-nitrophenol were followed by stopped-flow spectrophotometry with two 4-nitrophenyl ester substrates of penicillin G acylase from Kluyvera citrophila. With the ester of acetic acid, but not of propionic acid, there was a pre-steady-state exponential phase, the kinetics...
Topics
- Acylation
- Catalysis
- Enterobacteriaceae
- Hydrolysis
- Kinetics
- Mutation
- Nitrophenols
- Penicillin Amidase
- Penicillin G
- Phenylacetates
- Phenylpropionates
- Spectrophotometry