Article
[The pH-dependent catalytic reaction of penicillin G acylase and its mutants].
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica - 1 Nov 2002
Chen Jian-Bo, Yang Sheng, Wu Xing-Jia, Li Shi-Yun, Yuan Zhong-Yi
Abstract excerpt
The pH-dependence in the catalytic reaction of recombinant penicillin G acylase and its mutants from B.megaterium has been studied by using kinetic methods. pK(1) and pK(2)of the residues of the wild type penicillin G a cylase, involved in the catalyzed reaction, were 5.50-5.87 and 10.73, respectively, from the curves of logV(m) and log(V(m)/K(m)) versus pH. Results showed tha t the pK(1) and pK(2) values of...
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