Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Cloning, overexpression, crystallization and preliminary X-ray crystallographic analysis of a slow-processing mutant of penicillin G acylase from Kluyvera citrophila.

Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Aug 2013

Varshney Nishant Kumar, Ramasamy Sureshkumar, Brannigan James A, Wilkinson Anthony J, Suresh C G

Abstract excerpt

Kluyvera citrophila penicillin G acylase (KcPGA) has recently attracted increased attention relative to the well studied and commonly used Escherichia coli PGA (EcPGA) because KcPGA is more resilient to harsh conditions and is easier to immobilize for the industrial hydrolysis of natural penicillins to generate the 6-aminopenicillin (6-APA) nucleus, which is the starting material for semi-synthetic antibiotic...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Cloning, Molecular

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Crystallization

Share to Crystallography, X-Ray

Share to Gene Expression Regulation, Bacterial

Share to Mutation

Share to Protein Folding

Source

Open in PubMed Open full text

Identifiers

PMID: 23908045
DOI: 10.1107/S174430911301943X
PMCID: PMC3729176

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Cloning, overexpression, crystallization and preliminary X-ray crystallographic analysis of a slow-processing mutant of penicillin G acylase from Kluyvera citrophila.PMID 23908045Read ↗

Structure mediation in substrate binding and post-translational processing of penicillin acylases: Information from mutant structures of Kluyvera citrophila penicillin G acylase.Semantic proximity Read ↗

Changing glycine 21 for glutamic acid in the beta-subunit of penicillin G acylase from Kluyvera citrophila prevents protein maturation.Semantic proximity Read ↗

Crystallization and preliminary X-Ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16.Semantic proximity Read ↗

Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active-site cleft.Semantic proximity Read ↗

Thermodynamic profiles of penicillin G hydrolysis catalyzed by wild-type and Met----Ala168 mutant penicillin acylases from Kluyvera citrophila.Semantic proximity Read ↗

Crystal structures of penicillin acylase enzyme-substrate complexes: structural insights into the catalytic mechanism.Semantic proximity Read ↗

Expression, purification and crystallization of penicillin G acylase from Escherichia coli ATCC 11105.Semantic proximity Read ↗

Structural features of cephalosporin acylase reveal the basis of autocatalytic activation.Semantic proximity Read ↗

Rapid burst kinetics in the hydrolysis of 4-nitrophenyl acetate by penicillin G acylase from Kluyvera citrophila. Effects of mutation F360V on rate constants for acylation and de-acylation.Semantic proximity Read ↗

Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies.Semantic proximity Read ↗

Structure and function of a serine carboxypeptidase adapted for degradation of the protein synthesis antibiotic microcin C7.Semantic proximity Read ↗

Primary structure requirements for the maturation in vivo of penicillin acylase from Escherichia coli ATCC 11105.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.