Article
Acylation of porcine pancreatic phospholipase A2 influences penetration and substrate head-group binding, depending on the position of the acylated lysine in the enzyme molecule.
European journal of biochemistry - 1 Sept 1993
Lugtigheid R B, Nicolaes G A, Veldhuizen E J, Slotboom A J, Verheij H M, De Haas G H
Abstract excerpt
A porcine pancreatic phospholipase A2 mutant was constructed in which all nine lysines were replaced by arginines. The mutant displayed 68% residual activity on micellar zwitterionic substrates, indicating that lysines are not absolutely required for the catalytic action of the enzyme. Likewise, mutants with one single lysine present either at position 56, located close to the entrance of the active site, or at...
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