Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Changing glycine 21 for glutamic acid in the beta-subunit of penicillin G acylase from Kluyvera citrophila prevents protein maturation.

Applied microbiology and biotechnology - 1 Feb 1992

Prieto I, Rodríguez M C, Márquez G, Pérez-Aranda A, Barbero J L

Abstract excerpt

Active penicillin acylase from Kluyvera citrophila strain ATCC 21,285 consists of two different alpha- and beta-subunits derived from a single precursor by post-translational processing. Using the chemical mutagen hydroxylamine we have treated plasmid pYKD59 containing the active penicillin acylase gene (pga) from K. citrophila and have generated different point mutant penicillin acylase genes, one producing a...

Topics

Amino Acid Sequence
Chromosome Mapping
Enterobacteriaceae
Hydroxylamine
Hydroxylamines
Molecular Sequence Data
Mutagenesis
Mutagens
Mutation
Penicillin Amidase
Protein Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sequence Homology, Nucleic Acid

Source

Identifiers

PMID: 1368070
DOI: 10.1007/BF00183245

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1368070-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

Changing glycine 21 for glutamic acid in the beta-subunit of penicillin G acylase from Kluyvera citrophila prevents protein maturation.PMID 1368070Read ↗

Structure mediation in substrate binding and post-translational processing of penicillin acylases: Information from mutant structures of Kluyvera citrophila penicillin G acylase.Semantic proximity Read ↗

Cloning, overexpression, crystallization and preliminary X-ray crystallographic analysis of a slow-processing mutant of penicillin G acylase from Kluyvera citrophila.Semantic proximity Read ↗

Primary structure requirements for the maturation in vivo of penicillin acylase from Escherichia coli ATCC 11105.Semantic proximity Read ↗

Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active-site cleft.Semantic proximity Read ↗

Escherichia coli gamma-glutamyltranspeptidase mutants deficient in processing to subunits.Semantic proximity Read ↗

Thermodynamic profiles of penicillin G hydrolysis catalyzed by wild-type and Met----Ala168 mutant penicillin acylases from Kluyvera citrophila.Semantic proximity Read ↗

Expression, purification and crystallization of penicillin G acylase from Escherichia coli ATCC 11105.Semantic proximity Read ↗

Rapid burst kinetics in the hydrolysis of 4-nitrophenyl acetate by penicillin G acylase from Kluyvera citrophila. Effects of mutation F360V on rate constants for acylation and de-acylation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.