Article
Subcellular trafficking kinetics of GLU4 mutated at the N- and C-terminal.
The Biochemical journal - 1 Apr 1996
Araki S, Yang J, Hashiramoto M, Tamori Y, Kasuga M, Holman G D
Abstract excerpt
The glucose transporter isoform, GLUT4, has been expressed in Chinese hamster clones and its subcellular trafficking has been determined following labelling at the cell surface with the impermeant bis-mannose photolabel, 2-N-(1-azi-2,2,2-trifluoroethyl)benzoyl-1,3-bis(D-mannos -4-yloxy)-2-propylamine (ATM-BMPA). ATM-BMPA-tagged GLUT4 leaves the cell surface rapidly and equilibrates to give an internal/surface...
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