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Article

Asp-285 of the metal-tetracycline/H+ antiporter of Escherichia coli is essential for substrate binding.

FEBS letters - 10 Jun 1996

Kimura T, Yamaguchi A

Abstract excerpt

The transposon Tn10-encoded metal-tetracycline/H+ antiporter (TetA(B)) was preferentially photolabeled when [3H]tetracycline was irradiated in the presence of energized inverted membrane vesicles containing the TetA protein. The degree of labeling depended on the duration of irradiation and the energization of the membrane. Photolabeling was not observed in vesicles containing the Asp-285 --> Asn mutant TetA...

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Identifiers

PMID: 8654589
DOI: 10.1016/0014-5793(96)00514-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Asp-285 of the metal-tetracycline/H+ antiporter of Escherichia coli is essential for substrate binding.PMID 8654589Read ↗

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