Article
Effects of sulfhydryl reagents on the Cys65 mutant of the transposon Tn10-encoded metal-tetracycline/H+ antiporter of Escherichia coli.
FEBS letters - 10 May 1993
Yamaguchi A, Kimura T, Sawai T
Abstract excerpt
The Cys65 mutant of the Tn10-encoded metal-tetracycline/H+ antiporter is the only one which is inactivated by sulfhydryl reagents among the Cys mutants of the putative loop2-3 region [Yamaguchi, A. et al. (1992) J. Biol. Chem. 267, 19155-19162]. The tetracycline transport activity of the Cys65 mutant was completely abolished by N-ethylmaleimide; however, methyl methanethiosulfonate only abolished 45% of the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
