Article
Determination of a transmembrane segment using cysteine-scanning mutants of transposon Tn10-encoded metal-tetracycline/H+ antiporter.
Biochemistry - 10 Dec 1996
Kimura T, Suzuki M, Sawai T, Yamaguchi A
Abstract excerpt
The metal-tetracycline/H+ antiporter is a bacterial plasma membrane protein belonging to the 12-transmembrane transporter family. The boundaries of membrane-embedded domains can be clearly determined by means of a method involving cysteine-scanning mutants and their reactivity to N-ethylmaleimide...
Topics
- Amino Acid Sequence
- Antiporters
- Bacterial Proteins
- Cell Membrane
- Cysteine
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Ethylmaleimide
- Membrane Proteins
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Binding
- Tetracycline Resistance