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Article

Increased efficiency of alkaline phosphatase production levels in Escherichia coli using a degenerate PelB signal sequence.

Gene - 17 Apr 1996

Le Calvez H, Green J M, Baty D

Abstract excerpt

To obtain an expression vector that will optimize secretion of proteins with disulfide bridges in Escherichia coli, we fused the phoA gene, encoding the bacterial alkaline phosphatase (PhoA), to the sequence encoding the pectate lyase B signal sequence (PelBSS). We used an extensively degenerate pelBSS with silent mutations to study their effects on the production level and activity of PhoA. 11 representative...

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Identifiers

PMID: 8621088
DOI: 10.1016/0378-1119(95)00850-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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