Article
Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three-dimensional structure.
The Journal of biological chemistry - 15 Jan 1991
Hulett F M, Kim E E, Bookstein C, Kapp N V, Edwards C W, Wyckoff H W
Abstract excerpt
Bacillus subtilis has an alkaline phosphatase multigene family. Two members of this gene family, phoAIII and phoAIV, were cloned, taking advantage of in vitro constructed strains containing a plasmid insertion within one or the other of the structural genes. The DNA sequences of the two genes showed approximately 64% identity at the DNA level and 63% identity in the deduced primary amino acid sequences. The...
Topics
- Alkaline Phosphatase
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- Binding Sites
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Molecular Sequence Data
- Mutation