Article
Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli.
BMC biotechnology - 11 May 2020
Gawin Agnieszka, Ertesvåg Helga, Hansen Sine Alise Hartvigsen, Malmo Jostein, Brautaset Trygve
Abstract excerpt
BACKGROUND: Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, however, problems encountered in the periplasmic production that can be associated with the incorrect formation of disulfide bonds, incomplete cleavage of the signal peptide, and proteolytic...
Topics
- Bacterial Proteins
- DNA-Binding Proteins
- Endopeptidases
- Escherichia coli
- Escherichia coli Proteins
- Gene Editing
- Gene Expression Regulation, Bacterial
- Gene Knockout Techniques
- Membrane Proteins
- Molecular Chaperones
- Mutation
- Peptide Hydrolases